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- 2025年10月16日
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| 规格： | 100ug | 产品价格： | ￥1880.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥5680.0 |
| 规格： | 20ug | 产品价格： | ￥580.0 |
| 产品编号 | bs-41304P |
| 英文名称 | Recombinant mouse CD80 protein, N-GST & C-His |
| 中文名称 | 重组小鼠CD80蛋白 |
| 英文别名 | Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_MOUSE; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80; Recombinant mouse CD80 protein, N-GST & C-His |
| 性状 | Lyophilized or Liqui |
| 纯化方法 | AC |
| 理论分子量 | 50.1 |
| 浓度 | ＞0.5 mg/ml |
| 储存液 | 20mM Tris (pH8.0)with 150mM NaCl. |
| 研究领域 | Immunology > Adaptive Immunity > B Cells > CD Stem Cells > Hematopoietic Progenitors > Lymphoid > B Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Dendritic Cell Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Monocytic Lineage |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Expressed on activated B-cells, macrophages and dendritic cells. |
| 相似性 | Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. |
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文献和实验depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
expressing each construct (GST alone, GST fusion proteins) into individual 5-ml aliquots of LB broth containing appropriate antibiotic selection. Grow overnight at 37°C with shaking. Although variations of GST fusion protein expression vectors
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
Bodenmiller, B., Malmstrom, J., Gerrits, B., Campbell, D., Lam, H., Schmidt, A., Rinner, O., Mueller, L.N., Shannon, P.T., Pedrioli, P.G., Panse, C., Lee, H.K., Schlapbach, R., and Aebersold, R. 2007
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