- ￥1755 - 10998
- Biorbyt
- 英国
- orb1516765
- 2026年08月06日
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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
6 months from date of receipt.
- 英文名：
Recombinant mouse CD80 protein, N-GST & C-His
- 库存：
99
- 供应商：
Biorbyt
- 规格：
20 ug/100 ug/500 ug
| 规格： | 20 ug | 产品价格： | ￥1755.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥4680.0 |
| 规格： | 500 ug | 产品价格： | ￥10998.0 |
描述: Recombinant mouse CD80 protein, N-GST & C-His, with a concentration of >0.5 mg/ml, expressed in E.coli, with a molecular weight of 50.1 kDa, and a purity of >90% as determined by SDS-PAGE. It was purified by AC, and has a N-GST & C-His tag.
别名: Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_MOUSE; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80; Recombinant mouse CD80 protein, N-GST & C-His
浓度: >0.5 mg/ml
形态: Lyophilized or Liquid
靶点: T-lymphocyte activation antigen CD80
内毒素: Not analyzed
表达系统: E.coli
分子量: 50.1 kDa
防腐剂: 20mM Tris (pH8.0)with 150mM NaCl.
蛋白序列: 38-246/306
纯度: >90% as determined by SDS-PAGE
纯化方法: AC
复溶/重构: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
标签: N-GST & C-His
研究领域: Immunology & Inflammation
保存条件: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
备注: For research use only.
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文献和实验depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
5- If required, bring to 1pM RARE or any HRE you need 6- Incubate 30min at 4 _ C 7- Add 50_ L of the GST affinity matrix 8- Incubate for 2 hours at 4 _ C 9- Wash twice with PBS-DTT buffer (quick pulse to 500 x g) 10- Add 25_ L of SDS loading buffer directly
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