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| 产品编号 | bs-3666P |
| 英文名称 | Calmodulin 1 Antibody Blocking Peptide |
| 中文名称 | 钙调节素/钙调蛋白/钙调素封闭多肽 |
| 英文别名 | CALM 1; CALM 2; CALM 3; CALM; CALM1; CALM2; CALM3; CALML2; Calmodulin 1; Calmodulin1; CAM 1; CAM 2; CAM 3; CaM; CAM I; CAM1; CAM2; CAM3; CAMB; CAMC; CAMI; CAMIII; DD132; PHKD; Phosphorylase kinase delta; Phosphorylase kinase delta subunit; Calmodulin1; Calmodulin 1; Calmodulin-1; Calmodulin2; Calmodulin 2; Calmodulin-2; Calmodulin3; Calmodulin 3; Calmodulin-3; Calmodulin; CALM1_HUMAN. |
| 纯化方法 | HPLC |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates Cardiovascular > Atherosclerosis > Apoptosis Cardiovascular > Heart > Cardiac metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism Metabolism > Types of disease > Cancer Metabolism > Types of disease > Heart disease Signal Transduction > Signaling Pathway > Calcium Signaling > Calcium Binding Proteins Signal Transduction > Signaling Pathway > Calcium Signaling > Calmodulin Pathway |
| 亚基 | Interacts with MYO1C and RRAD. Interacts with MYO10 (By similarity). Interacts with CEP97, CEP110, TTN/titin and SRY. Interacts with USP6; the interaction is calcium dependent. Interacts with CDK5RAP2. Interacts with SCN5A. Interacts with RYR1 and RYR2. |
| 亚细胞定位 | Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. |
| 翻译后修饰 | Ubiquitination results in a strongly decreased activity. _x000D_ Phosphorylation results in a decreased activity. |
| 相似性 | Belongs to the calmodulin family. Contains 4 EF-hand domains. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Calmodulin consists of two glycoproteins, 34 and 39 kDa, sometimes designated epithelial antigen, epithelial specific antigen, and epithelial glycoprotein. The glycoproteins are located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells with the exception of most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells. Epithelial Calmodulin is found in the large majority of adenocarcinomas of most sites (50-100% in various studies; as well as neuroendocrine tumours, including small cell carcinoma. Renal cell carcinoma and hepatocellular carcinoma stain in about 30% of the cases. Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin Ca(2+) complex are a number of protein kinases and phosphatases. Calmodulin has four functional calcium binding sites. |
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文献和实验Synthesis and Probing of Membrane-bound Peptide Arrays
a minimum of 50 µl. Consider, for example, a list of peptides that requires alanine for 26 peptides at cycle 1 on a 17 x 25 array. This cycle will require 26 (peptides) x 0.2 (µl per elongation) x 3 (couplings) = 15.6 µl of Fmoc-Ala stock solution
Bromodeoxyuridine Immunohistochemistry
solution. C. Immunohistochemistry using an avidin/biotin/peroxidase complex (ABC) 12. Warm slides at least 45 min., RT 13. 1X PBS, 3 min. X 5, RT. 14. Incubate all slides with secondary Ab 1 hr, RT. Prepare by adding 1 drop normal horse
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
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