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- 库存:
88
- 规格:
20mg
| Product Name | 740 Y-P (Synonyms: 740YPDGFR; PDGFR 740Y-P) |
| Cat. No. | TD-HYP0175 |
| Description | 740 Y-P (740YPDGFR; PDGFR 740Y-P) 是一个有效的,具有细胞渗透性的 PI3K 激活剂。740 Y-P 很容易结合含有 p85 的 N- 和 C- 末端 SH2 结构域的 GST 融合蛋白,但不能单独结合 GST。 |
| Purity | 99.96% |
| CAS No. | 1236188-16-1 |
| 分子式 | C₁₄₁H₂₂₂N₄₃O₃₉PS₃ |
| 分子量 | 3270.7 |
| Sequence | Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-{Tyr(PO₂)}-Met-Asp-Met-Ser |
| Sequence Shortening | RQIKIWFQNRRMKWKKSDGG-{Tyr(PO₂)}-MDMS |
| 作用靶点 | PI3K; Autophagy |
| 作用通路 | PI3K/Akt/mTOR; Autophagy |
| 储存方式 | Sealed storage, away from moisture and light Powder:-80°C, 2 years; -20°C, 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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文献和实验chloramphenicol and cultured at 37°C with shaking for 16hrs. The liquid bacterial culture is transferred into the tube specific for AutoGene 740 DNA Isolation System (Autogen, Inc. ) 2、For BAC DNA purification, we use alkaline lysis approach by set
Preparation of Carboxypeptidase Y and Its Properties
while high efficiency habit in the scientific research. 【Requirement】 1. Use bread yeast as the original material to extract and condense carboxypeptidase Y, assay its biochemical properties. 2. Through gathering relevant published papers
yzzaici888 大家好,本人最近一直在做p-p38的磷酸化,但是一直都做不好。希望各位大侠帮助解决: 蛋白上样量80-100ug,半干转膜70min,5%BSA封闭70min,一抗CST(#9211) 1:500加,4℃孵育过夜,二抗1:8000加室温70min。曝光出的结果很不好,杂带很多,也分析不出那条是目的条带,且没有趋势,见下图 15001046419
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