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- IHC0536
- 2025年10月15日
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| 产品编号 | IHC0536 |
| 英文名称 | PLIN2 Ready-To-Use IHC Kit |
| 中文名称 | 脂肪分化相关蛋白即用型免疫组化试剂盒 |
| 英文别名 | AA407157; ADPH; ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; PLIN2; PLIN2_HUMAN; adipophilin; Adipose differentiation related protein; ADRP; MGC10598 |
| 产品应用 | IHC-P Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 亚基 | Interacts with IRGC. |
| 亚细胞定位 | #Lipid droplet #Membrane |
| 组织特异性 | Milk lipid globules. |
| 翻译后修饰 | Acylated; primarily with C14, C16 and C18 fatty acids.2 Publications Phosphorylation at Tyr-232 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis.1 Publication Polyubiquitination of Nt-acetylatable A-PLIN2 by MARCHF6 lead to degradation by 26S proteasomes. |
| 相似性 | Belongs to the perilipin family. |
| 功能 | Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets. |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 背景资料 | Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells. |
| 应用 | 推荐稀释比例 |
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文献和实验New double-positivity tool for multi-color IHC IF
by and reflected in local microenvironments, as well as how diseased cells respond to therapies. These kinds of questions can be answered in a limited way by methods that disaggregate or homogenize tissue, or that utilize conventional single-stain IHC
Imaging Techniques in Signal Transduction IHC
values within the dynamic range of the detector, frame averaging to eliminate noise (typically in fluorescence imaging), use of the highest bit depth when a choice is available, flatfield correction, and archiving of the image in a nonlossy format
的染色强度。获得合适的染色强度后，将载玻片置于蒸馏水 (dH2O) 中以阻止进一步显色。抗体：Phospho–Stat3(Tyr705)(D3A7) XP® Rabbit mAb #9145样本：石蜡包埋的人类乳腺肿瘤显色检测：利用 SignalStain® DAB Substrate Kit #8059（左），竞争者提供的 DAB（中和右）CST 的 IHC/IF 明星产品推荐：生化试剂：Catalog NumberProduct NameProduct
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