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- 2025年10月15日
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| 产品编号 | IHC0443H |
| 英文名称 | Human MtTF1 Ready-To-Use IHC Kit |
| 中文名称 | 人线粒体转录因子1即用型免疫组化试剂盒 |
| 英文别名 | AI661103; Hmgts; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; tsHMG; MTDPS15 |
| 产品应用 | IHC-P Not yet tested in other applications. |
| 交叉反应 | Human |
| 亚基 | Monomer; binds DNA as a monomer (PubMed:19304746, PubMed:22037171, PubMed:22037172). Homodimer (PubMed:29149603). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT (PubMed:29149603). In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:29149603). Upon metabolic stress, forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT (PubMed:12897151, PubMed:29445193). Interacts with TFB1M and TFB2M (PubMed:12897151). Interacts with CLPX; this enhances DNA-binding (PubMed:22841477). |
| 亚细胞定位 | #Mitochondrion #Mitochondrion nucleoid |
| 翻译后修饰 | Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease. |
| 功能 | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed:19304746, PubMed:22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171). |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 背景资料 | Mitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes, but it is transported from the cytoplasm to mitochondria where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates the replication and transcription of mtDNA, ensuring the proper functioning of mitochondrial processes. Structurally, TFAM contains a molecular region known as the LC3 interacting region (LIR) motif, which allows it to bind to autophagy proteins such as LC3. This interaction is essential for the autolysosomal pathway, helping to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, with notable effects on mitochondrial function; high levels can repress mtDNA expression in certain tissues, such as skeletal muscle, and contribute to deficiencies in oxidative phosphorylation (OXPHOS). Conversely, in other tissues like the heart, increased mtDNA copy number maintains a balanced TFAM-to-mtDNA ratio, preserving OXPHOS capacity. TFAM is integral to the packaging, stability, and replication of the mitochondrial genome, and disruptions in TFAM can have severe effects, including implications in neurodegeneration and other diseases related to mtDNA stress. |
| 应用 | 推荐稀释比例 |
Immunohistochemical analysis of paraffin embedded human lung cancer tissue slide using IHC0443H (Human MtTF1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human testis tissue slide using IHC0443H (Human MtTF1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human thyroid carcinoma tissue slide using IHC0443H (Human MtTF1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human kidney tissue slide using IHC0443H (Human MtTF1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human esophagus tissue slide using IHC0443H (Human MtTF1 IHC Kit).
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Human MtTF1 Ready-To-Use IHC Kit(IHC0443H)-50T
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