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| 产品编号 | IHC0114R |
| 英文名称 | Rat alpha smooth muscle Actin Ready-To-Use IHC Kit |
| 中文名称 | 大鼠肌动蛋白α/α-SMA/α Actin单克隆即用型免疫组化试剂盒 |
| 英文别名 | alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5 |
| 产品应用 | IHC-P Not yet tested in other applications. |
| 交叉反应 | Rat |
| 研究领域 | Cardiovascular > Cardiovascular Markers > Cell Markers > Smooth Muscle Cells Cardiovascular > Heart > Contractility > Contractile Proteins > Actins Cardiovascular > Vasculature > Smooth muscle cell (SMC) > Cytoskeleton Cardiovascular > Vasculature > Vasculature Markers > Arterial Developmental Biology > Lineage specification > Mesoderm Signal Transduction > Antibodies > alpha smooth muscle Actin Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Stem Cells > Lineage Markers > Mesoderm Stem Cells > Mesenchymal Stem Cells > Myogenesis |
| 亚基 | Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 翻译后修饰 | Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced (By similarity). |
| 相似性 | Belongs to the actin family. |
| 功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha-Actin expression is limited to various types of muscle, whereas beta- and gamma-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia. |
| 应用 | 推荐稀释比例 |
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文献和实验【原创】转载Western Blot 内参选择攻略（美季生物技术有限公司）http://www.majorbio.cn/NewsInfo.asp?id=289
-tubulin。一般我们选择内参与要检测的目的蛋白的分子量最好相差5KD以上。因此你要知道你检测的蛋白的分子量来选择合适的内参！ actin即肌动蛋白，是细胞的一种重要骨架蛋白。actin大致可分为六种，其中四种是不同肌肉组织特异性的，包括alpha-skeletal muscle actin、alpha-cardiac muscle actin、alpha-smooth muscle actin和gamma-smooth muscle actin，其余两种广泛分布于各种组织中，包括beta-actin(β
Endothelial Cell Systems - Instructions For Use
cells test Positive for von Willebrand Factor VIII and Acetylated LDL and test Negative for Alpha Smooth Muscle Actin. 4.Inaddition to the above staining, HMVEC-L also test positively for platelet endothelial cell adhesion molecule (PECAM
Choi,B.M., Kim,Y.M., Jeong,Y.R., Pae,H.O., Song,C.E., Park,J.E., Ahn,Y.K. and Chung,H.T. TITLE Induction of heme oxygenase-1 is involved in anti-proliferative effects of paclitaxel on rat vascular smooth muscle cells JOURNAL Biochem. Biophys. Res
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