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| 规格: | 20ug | 产品价格: | ¥980.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥1880.0 |
| 规格: | 100ug | 产品价格: | ¥2680.0 |
| 产品编号 | bs-104811P |
| 英文名称 | Recombinant Human TRIP11 Protein, N-His |
| 英文别名 | TRIP-11; CEV14; TR-interacting protein 11; Thyroid receptor-interacting protein 11; Golgi-associated microtubule-binding protein 210; Clonal evolution-related gene on chromosome 14 protein; Trip230; TRIP11; GMAP-210 |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 17.09 kDa |
| 储存液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Co-activators/co-repressors Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Co-activators/co-repressors |
| 亚细胞定位 | Cell Membrane and Golgi Apparatus |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | TRIP230 was identified through its ability to interact functionally with thyroid hormone receptor-beta. It has also been found in association with the Golgi apparatus and microtubules. TRIP230 binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription. This protein is probably involved in maintaining cis-Golgi structure. |
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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS (DEPC处理的)含50%甲酰胺的杂交液(HS)--- 1ml分装后置-20℃保存 10×阻断液(10×BS) Buffer I Buffer II Buffer III 探针(P) 抗Dig-Ap NBT/BCIP 二、操作流程: 1) 脱蜡至水: 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2) 杂交前处理: 1×PBS洗涤5min → 蛋白酶K(终浓度20ug/ml in PBS)37℃
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