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| 规格： | 20ug | 产品价格： | ￥1280.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥2580.0 |
| 规格： | 100ug | 产品价格： | ￥4180.0 |
| 产品编号 | bs-104732P |
| 英文名称 | Recombinant Human TNFSF9/4-1BBL Protein, C-His |
| 英文别名 | Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; TNFSF9 |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 23 kDa |
| 储存液 | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 研究领域 | Cancer > Growth factors > TNF Immunology > Adaptive Immunity > T Cells > Cytotoxic Cells Immunology > Innate Immunity > Cytokines > TNF Superfamily |
| 亚基 | Homotrimer. |
| 亚细胞定位 | Membrane; Single-pass type II membrane protein. |
| 组织特异性 | Expressed in brain, placenta, lung, skeletal muscle and kidney. |
| 相似性 | Belongs to the tumor necrosis factor family. |
| 功能 | Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This cytokine belongs to the tumour necrosis factor (TNF) ligand family. It is a ligand for TNFRSF9, which is a costimulatory molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process, and in the generation of cytotoxic T cells. This cytokine is also shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is found to be expressed in various carcinoma cell lines, and is thought to be involved in T cell-tumour cell interaction. |
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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS （DEPC处理的）含50%甲酰胺的杂交液（HS）--- 1ml分装后置-20℃保存 10×阻断液（10×BS） Buffer I Buffer II Buffer III 探针（P） 抗Dig-Ap NBT/BCIP 二、操作流程: 1） 脱蜡至水： 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2） 杂交前处理： 1×PBS洗涤5min → 蛋白酶K（终浓度20ug/ml in PBS）37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
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