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- bs-101922P
- 2025年10月15日
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| 规格： | 20ug | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥1880.0 |
| 规格： | 100ug | 产品价格： | ￥2680.0 |
| 产品编号 | bs-101922P |
| 英文名称 | Recombinant Human MFAP5 Protein, N-His |
| 英文别名 | MAGP2; MAGP-2; MFAP-5; MP25; Microfibril-associated glycoprotein 2; MFAP5; Microfibrillar-associated protein 5 |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 18.16 kDa |
| 储存液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 研究领域 | Cancer > Invasion/microenvironment > Angiogenesis > Angiogenic growth factors Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Proteins > Elastin |
| 亚细胞定位 | Secreted, extracellular space, extracellular matrix. |
| 翻译后修饰 | Forms intermolecular disulfide bonds either with other MAGP-2 molecules or with other components of the microfibrils. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. O-glycan heterogeneity at Thr-54: HexHexNAc (major) and HexHexNAc + sulfate (minor). |
| 相似性 | Belongs to the MFAP family. |
| 功能 | Component of the elastin-associated microfibrils. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | MAGP2 (also known as Microfibril-associated glycoprotein 2, MFAP2, MP25 and Microfibrillar-associated protein 5 precursor) is a secreted, extracellular matrix protein which is a component of the elastin-associated microfibrils. It forms intermolecular disulfide bonds either with other MAGP2 molecules or with other components of the microfibrils. MAGP2 is upregulated in some ovarian cancers and is associated with poor survival of ovarian cancer patients. |
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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS （DEPC处理的）含50%甲酰胺的杂交液（HS）--- 1ml分装后置-20℃保存 10×阻断液（10×BS） Buffer I Buffer II Buffer III 探针（P） 抗Dig-Ap NBT/BCIP 二、操作流程: 1） 脱蜡至水： 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2） 杂交前处理： 1×PBS洗涤5min → 蛋白酶K（终浓度20ug/ml in PBS）37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
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