- ￥980 - 2680
- Bioss
- bs-100820P
- 2025年10月15日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 规格：
20ug/50ug/100ug
| 规格： | 20ug | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥1880.0 |
| 规格： | 100ug | 产品价格： | ￥2680.0 |
| 产品编号 | bs-100820P |
| 英文名称 | Recombinant Human PDIA4 Protein, N-His |
| 英文别名 | ERp-72; ER protein 70; ER protein 72; ERP70; Protein disulfide-isomerase A4; ERP72; PDIA4; Endoplasmic reticulum resident protein 72; ERp72; ERp70; Endoplasmic reticulum resident protein 70 |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 17.09 kDa |
| 储存液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 研究领域 | Signal Transduction > Protein Trafficking > Chaperones > Other Chaperones Signal Transduction > Protein Trafficking > ER Proteins |
| 亚基 | Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. |
| 亚细胞定位 | Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 相似性 | Belongs to the protein disulfide isomerase family. Contains 3 thioredoxin domains. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Mammals defend themselves against intracellular pathogens through presentation of cytoplasmically derived short pathogenic peptides to the cell surface of cytotoxic T lymphocytes, which subsequently leads to cytotoxic events with respect to the affected cell. Antigen presentation is mediated by major histocompatibility complex (MHC) class I molecules, which bind and coordinate short pathogenic peptides. The proper folding and assembly of MHC class I molecules in the endoplasmic reticulum (ER) involve a number of components. MHC class I molecules assemble in the ER with chaperones before binding to the transporter associated with antigen processing (TAP) protein. ERp57 is a component of the MHC class I pathway that appears to interact with MHC class I molecules before they associate with TAP. ERp72, also designated protein disulfide-isomerase A4, is involved in the catalysis of protein -S-S- bond rearrangement. ERp57 and ERp72 may act as proteases, protein disulfide isomerases, phospholipases or a combination of these. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS （DEPC处理的）含50%甲酰胺的杂交液（HS）--- 1ml分装后置-20℃保存 10×阻断液（10×BS） Buffer I Buffer II Buffer III 探针（P） 抗Dig-Ap NBT/BCIP 二、操作流程: 1） 脱蜡至水： 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2） 杂交前处理： 1×PBS洗涤5min → 蛋白酶K（终浓度20ug/ml in PBS）37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
技术资料暂无技术资料 索取技术资料
