Blank control:A431.
Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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- bs-10011R-FITC
- 2025年10月15日
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100ul
| 产品编号 | bs-10011R-FITC |
| 英文名称 | Occludin Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的紧密连接蛋白抗体 |
| 英文别名 | Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin. |
| 产品应用 | Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Occludin |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Tight Junctions |
| 亚基 | Interacts with TJP1/ZO1 and with VAPA. |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. Cell junction, tight junction. |
| 组织特异性 | Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts. |
| 相似性 | Belongs to the ELL/occludin family.
Contains 1 MARVEL domain. |
| 功能 | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg/Test} |
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Occludin Rabbit pAb, FITC conjugated(bs-10011R-FITC)-100ul
￥2980
