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- bs-6528R-PerCP-Cy5.5
- 2025年09月30日
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100ul
| 产品编号 | bs-6528R-PerCP-Cy5.5 |
| 英文名称 | EZH1 Rabbit pAb, PerCP-Cy5.5 conjugated |
| 中文名称 | PerCP-Cy5.5标记的组蛋白赖氨酸N-甲基EZH1抗体 |
| 英文别名 | Enhancer of zeste homolog 1(Drosophila); Enhancer of zeste homolog 1; ENX-2; ENX2; EZH1; EZH1: enhancer of zeste homolog 1(Drosophila); EZH1_HUMAN; Histone-lysine N-methyltransferase EZH1; KIAA0388. |
| 产品应用 | Flow-Cyt=2ug/Test, ICC/IF=1:50-200, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human EZH1 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA and 0.02% Proclin300. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Methylation Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Methylation > Lysine methylation Epigenetics and Nuclear Signaling > Chromatin Remodeling > Polycomb Silencing > PRC2 |
| 亚基 | Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2/EED-EZH1 is less abundant than the PRC2/EED-EZH2 complex, has weak methyltransferase activity and compacts chromatin in the absence of the methyltransferase cofactor S-adenosyl-L-methionine (SAM). |
| 亚细胞定位 | Nucleus. Note=Colocalizes with trimethylated 'Lys-27' of histone H3. |
| 相似性 | Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 CXC domain. Contains 1 SET domain. |
| 功能 | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. |
| 保存条件 | Store at 2-8°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. Sequence similarities Belongs to the histone-lysine methyltransferase family. EZ subfamily.Contains 1 SET domain. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2ug/Test} |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:100-500} |
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文献和实验Fluorochrome Absorption and Emission Spectra
. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on stream-in-air flow cytometers. PerCP-Cy5.5 is a tandem conjugate system than combines PerCP with a cyanine dye (Cy5.5™) and has an absorption maximum
Fluorochrome Absorption and Emission
. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on stream-in-air flow cytometers. PerCP-Cy5.5 is a tandem conjugate system than combines PerCP with a cyanine dye (Cy5.5™) and has an absorption maximum
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