- ￥2980
- Bioss
- bs-3314R-PerCP-Cy5.5
- 2025年09月30日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
Annexin A1 Rabbit pAb, BF555 conjugated(bs-1562R-BF555)-100ul
￥2980
CD239 Rabbit pAb(bs-7546R)-50ul/100ul/200ul
￥1180
phospho-Caseine Kinase 1 alpha(Tyr294) Rabbit pAb(bs-12428R)-50ul/100ul/200ul
￥1180TRIP Rabbit pAb(bs-4983R)-50ul/100ul/200ul
￥1180
TSLC1 Rabbit pAb, PE conjugated(bs-6026R-PE)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bs-3314R-PerCP-Cy5.5 |
| 英文名称 | phospho-PAK6 (Ser560) Rabbit pAb, PerCP-Cy5.5 conjugated |
| 中文名称 | PerCP-Cy5.5标记的磷酸化p21激活激酶6抗体 |
| 英文别名 | phospho-PAK6(Ser560); PAK4+PAK5+PAK6(phospho S474+S602+S560); CDKN1A activated kinase 6; Serine/threonine-protein kinase PAK 6; p21 activated protein kinase 6; p21 protein(Cdc42/Rac)-activated kinase 6; p21(CDKN1A) activated kinase 6; p21-ACTIVATED KINASE 6; p21activated kinase 6; PAK 5; PAK 6; PAK5; Serine threonine protein kinase PAK 6; Serine/threonine protein kinase PAK 6; Serine/threonine protein kinase PAK6; EC 2.7.1.37; PAK-5; PAK-6; Pak6; PAK6_HUMAN. |
| 产品应用 | ICC/IF=1:100-500, Flow-Cyt=1ug/Test, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human PAK6 around the phosphorylation site of Ser560 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA and 0.02% Proclin300. |
| 研究领域 | Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 亚基 | Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with the androgen receptor AR and the estrogen receptor ESR1. Interacts with IQGAP1 and PPM1B. |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=Cotranslocates into nucleus with AR in response to androgen induction. |
| 组织特异性 | Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast. |
| 翻译后修饰 | Autophosphorylated. Phosphorylated by MAP2K6//MAPKK6, leading to PAK6 activation. |
| 相似性 | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily._x000D_
Contains 1 CRIB domain. |
| 功能 | Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD. |
| 保存条件 | Store at 2-8°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a protein that shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. The p21-activated protein kinase 6 gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
| {IF} | {1:100-500} |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
技术资料暂无技术资料 索取技术资料
