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产品信息以Bioss网站为准
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100ul
| 产品编号 | bs-13445R-Bio |
| 英文名称 | G3PP Rabbit pAb, Biotin conjugated |
| 中文名称 | Biotin标记的转运蛋白G3PP抗体 |
| 英文别名 | glycerol-3-phosphate permease; glycerol-3-phosphate; G-3-P permease; G-3-P transporter; G3P permease; G3P transporter; G3PP; GLPT_HUMAN; Glycerol-3-phosphate permease; Glycerol-3-phosphate transporter; SLC37A1; solute carrier family 37(glycerol-3-phosphate transporter), member 1; solute carrier family 37 member 1. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, ELISA=1:5000-10000, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human G3PP/SLC37A1 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Membrane. |
| 组织特异性 | Expressed in numerous tissues, with highest expression in kidney, bone marrow, spleen, liver, small intestine, as well as in fetal brain, liver and spleen. |
| 相似性 | Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | G3PP is a 533 amino acid protein belonging to the major facilitator superfamily. G3PP is a multi-pass membrane protein that acts as a transporter of or glycerol-3-phoshphate between cellular compartments. Glycerol-3-phosphate is produced from glycerol and is an important factor in many biochemical pathways, including glycolysis. G3PP contains 12 putative transmembrane domains and N- and C-terminal Endoplasmic Reticulum (ER) retention signals. G3PP is expressed at highest levels in liver, kidney, small intestine, bone marrow and fetal tissues. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
| {IF} | {1:100-500} |
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 0.2 μg/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems
of DNA?either natural or formed from oligonucleotides?containing a high?affinity site for a DNA?binding protein provide a powerful tool for purification. The biotin/streptavidin purification system is based on the tight and essentially irreversible
- or triple-labeling immunofluorescence, it is necessary to use primary antibodies raised in different host species such as mouse, rabbit, and goat. However, in many cases, suitable primary antibodies raised in different species are unavailable
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