RNA polymerase II CTD repeat Y
SPTSPS (phospho S2) Rabbit pAb, BF680 conjugated(bs-6581R-BF680)-100ul- ￥2980
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- bs-6581R-BF680
- 2025年10月27日
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100ul
| 产品编号 | bs-6581R-BF680 |
| 英文名称 | RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rabbit pAb, BF680 conjugated |
| 中文名称 | BF680标记的磷酸化RNA聚合酶II CTD抗体 |
| 英文别名 | Rpb1 CTD; RNA polymerase II; RNAPII; POLR2A; POLR2; DNA directed RNA polymerase II A; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; Polr2a; RNA pol II CTD; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPB1_HUMAN. |
| 产品应用 | Flow-Cyt=1ug/Test, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human RNA polymerase II CTD around the phosphorylation site of Ser2 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Polymerase associated factors > Pol II Transcription Epigenetics and Nuclear Signaling > Transcription > Polymerase associated factors > Pol II Transcription > Polymerase Epigenetics and Nuclear Signaling > Transcription > RNA polymerase |
| 亚基 | Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C (By similarity). Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2, CTDSP1 and SCAF8. Interacts via the phosphorylated C-terminal domain with WDR82 and with SETD1A and SETD1B only in the presence of WDR82. Interacts with ATF7IP. When phosphorylated at 'Ser-5', interacts with MEN1; the unphosphorylated form, or phosphorylated at 'Ser-2' does not interact. Interacts with DDX5. |
| 亚细胞定位 | Nucleus. |
| 翻译后修饰 | Dephosphorylated by the protein phosphatase CTDSP1. [PTM] Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity). [PTM] Methylated at Arg-1810 by CARM1. Methylation occurs only when the CTD is hypophosphorylated, and phosphorylation at Ser-1805 and Ser-1808 prevent methylation (in vitro). It is assumed that methylation occurs prior to phosphorylation and transcription initiation. CTD methylation may facilitate the expression of select RNAs. |
| 相似性 | Belongs to the RNA polymerase beta' chain family. |
| 功能 | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
| {IF} | {1:100-500} |
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文献和实验相关实验
from each cell culture condition. • Performing a ChIP using the Normal Rabbit IgG is an effective negative control. • The included anti-RNA polymerase II antibody and GAPDH primers provide a positive control for assay technique and reagent integrity
的染色质作为阳性对照管样本，然后加入与目的蛋白的 ChIP 抗体量相同的 H3 组蛋白抗体（CST 推荐的阳性对照抗体）或者 RNA polymerase II 抗体，进行后续沉淀反应。阳性对照管选择的抗体，常常在整个染色质结合位点丰富，用于检测实验操作是否正确，对于初次进行 ChIP 实验的操作者推荐使用。上述各样品管加入抗体后，用封口膜封紧管盖，在 4°C 的转子上（可置于翻转摇床上，转速 100~150）孵育 4 小时以上或过夜。注意：ChIP 抗体的特异性对于 ChIP 结果至关重要，决定
secondary antibody review -- data from 99 publications
whether any cell surface DFz2 had been internalized 4 flow cytometry localize of GPR30 in the endoplasmic reticulum 5 rhodamine immunocytochemistry detect kaposin B in SLK endothelial cells 6 IgG IgG2b Alexa Fluor 647 flow
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RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rabbit pAb, BF680 conjugated(bs-6581R-BF680)-100ul
￥2980
