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| 产品编号 | bs-19613P |
| 英文名称 | SEC23 Antibody Blocking Peptide |
| 中文名称 | 转运蛋白SEC23封闭多肽 |
| 英文别名 | CLSD; Protein transport protein Sec23A; SC23A_HUMAN; Sec23 homolog A (S. cerevisiae); SEC23-related protein A; sec23a. |
| 纯化方法 | HPLC |
| 亚细胞定位 | Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles. |
| 相似性 | Belongs to the SEC23/SEC24 family. SEC23 subfamily. |
| 功能 | Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family. It is part of a protein complex and found in the ribosome-free transitional face of the endoplasmic reticulum (ER) and associated vesicles. This protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The encoded protein is suggested to play a role in the ER-Golgi protein trafficking. [provided by RefSeq, Jul 2008] |
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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS (DEPC处理的)含50%甲酰胺的杂交液(HS)--- 1ml分装后置-20℃保存 10×阻断液(10×BS) Buffer I Buffer II Buffer III 探针(P) 抗Dig-Ap NBT/BCIP 二、操作流程: 1) 脱蜡至水: 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2) 杂交前处理: 1×PBS洗涤5min → 蛋白酶K(终浓度20ug/ml in PBS)37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
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