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- 2025年10月15日
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500ug
| 产品编号 | bs-6265P |
| 英文名称 | NEDD9 Antibody Blocking Peptide |
| 中文名称 | 蛋白激酶底物相关蛋白封闭多肽 |
| 英文别名 | CAS L; Cas like docking; Cas scaffolding protein family member 2; CAS-L; CAS2; CASL; CASL_HUMAN; CASS2; Crk associated substrate related protein; CRK-associated substrate-related protein; dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); Enhancer of filamentation 1; Enhancer of filamentation 1 p55; HEF 1; HEF1; NEDD 9; NEDD-9; NEDD9; NEDD9 protein; Neural cell expressed developmentally down regulated 9; Neural precursor cell expressed developmentally down regulated 9; Neural precursor cell expressed developmentally down-regulated protein 9; NY REN 12 antigen antibody p105; Protein NEDD9; Renal carcinoma antigen NY-REN-12. |
| 纯化方法 | HPLC |
| 研究领域 | Cancer > Signal transduction > Protein phosphorylation > Tyrosine kinases > FAK family Cell Biology > Apoptosis > Intracellular > Associated Proteins Epigenetics and Nuclear Signaling > Transcription > Other factors |
| 亚基 | Interacts with BCAR3 and SH2D3C. Homodimer. Can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas. Forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase. Interacts with MICAL and TXNL4/DIM1. |
| 亚细胞定位 | Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Note=Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle. Enhancer of filamentation 1 p55: Cytoplasm, cytoskeleton, spindle. |
| 组织特异性 | Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues. |
| 翻译后修饰 | Cell cycle-regulated processing produces four isoforms: p115,p105, p65, and p55. Isoform p115 arises from p105 phosphorylationand appears later in the cell cycle. Isoform p55 arises from p105as a result of cleavage at a caspase cleavage-related site and itappears specifically at mitosis. The p65 isoform is poorlydetected. Focal adhesion kinase 1 phosphorylates the protein at theYDYVHL motif (conserved among all cas proteins). The SRC familykinases (FYN, SRC, LCK and CRK) are recruited to the phosphorylatedsites and can phosphorylate other tyrosine residues. Ligation ofeither integrin beta-1 or B-cell antigen receptor on tonsillarB-cells and B-cell lines promotes tyrosine phosphorylation and bothintegrin and BCR-mediated tyrosine phosphorylation requires anintact actin network. In fibroblasts transformation with oncogenev-ABL results in an increase in tyrosine phosphorylation.Transiently phosphorylated following CD3 cross-linking and thisphosphorylated form binds to CRK and C3G. A mutant lacking the SH3domain is phosphorylated upon CD3 cross-linking but not uponintegrin beta-1 cross-linking. Tyrosine phosphorylation occurs uponstimulation of the G-protein coupled C1a calcitonin receptor inrabbit. Calcitonin-stimulated tyrosine phosphorylation is mediatedby calcium- and protein kinase C-dependent mechanisms and requiresthe integrity of the actin cytoskeleton. |
| 相似性 | Belongs to the CAS family.
Contains 1 SH3 domain. |
| 功能 | Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. |
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