- ￥5800
- Proteintech
- 60542-1-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PDGFRB protein
- 亚型：
IgG2a
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
Cytometric bead array, Indirect ELISA
- 靶点：
PDGFR beta/CD140b
- 抗体英文名：
PDGFR beta/CD140b Monoclonal antibody, PBS Only (Capture)
- 抗体名：
PDGFR beta/CD140b Monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
60542-1-PBS targets PDGFR beta/CD140b as part of a matched antibody pair:
MP50756-5: 60542-1-PBS capture and 60543-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag4285 Product name: Recombinant human PDGFRB protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 755-1106 aa of BC032224 Sequence: LDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | platelet-derived growth factor receptor, beta polypeptide |
| 别名 | PDGFRB, EC:2.7.10.1, CD140B, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor |
| 计算分子量 | 1106 aa, 124 kDa |
| GenBank蛋白编号 | BC032224 |
| 基因名称 | PDGFR beta |
| Gene ID (NCBI) | 5159 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09619 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Immuno-Laser Capture Microdissection
. Single-step PCR is able to amplify fragments of more than 600 bp from both housekeeping genes, e.g., beta-actin, as well as cell-specific messages, e.g., CD4 or CD19, using cDNA derived from less than 500 immunostained, microdissected cells
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
: Hydrodynamic properties of the complex prepared by a refined hydroxyapatite chromatography in Trition X‐100. Biochim. Biophys. Acta. 462:549‐558. Wilchek, M. and Jakoby, W.B. 1974
107 的 PBMC 细胞用 100μl 的 Buffer 稀释（Buffer CI：含 0.5% BSA 的 PBS 缓冲液，1 mM EDTA） · 加入多种生物素化抗体，室温下孵育 5 分钟。生物素化的抗体包含以下几种，每种抗体 0.5 μg：CD235a (erythrocytes), CD14 (monocytes), CD3 (T cells), CD15 (granulocytes), CD19 (B cells), CD36 (thrombocytes), CD123
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