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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG2a, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
FITC Plus Fluorescent Dye
- 适应物种:
mouse
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rat
- 应用范围:
FC
- 靶点:
CD4
- 抗体英文名:
FITC Plus Anti-Mouse CD4 (RM4-5)
- 抗体名:
FITC Plus Anti-Mouse CD4 (RM4-5)
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1200.0 |
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
FITC-65141 targets CD4 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 文献引用应用 | FC |
| 经测试反应性 | mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD4 antigen |
| 别名 | CD4 antigen, L3T4, Ly 4, RM4-5, T-cell differentiation antigen L3T4 |
| GenBank蛋白编号 | BC039137 |
| 基因名称 | Cd4 |
| Gene ID (NCBI) | 12504 |
| RRID | AB_2883801 |
| 偶联类型 | FITC Plus Fluorescent Dye |
| 最大激发/发射波长 | 495 nm / 524 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P06332 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
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|---|---|---|
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| mouse | FC | Int J Biol Macromol Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunizationAuthors - Kai Deng |
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光素结合蛋白峰,测定F/P 比值。第二个荧光素峰为游离荧光素 计算: 2.87×A495 F/P=──────── A280-0.35×A495 合适的F/P值为2~4。 (三) 试剂器材 1. 纯化的多克隆抗体或单克隆抗体。 2. FITC(Fluorescein-5-Lsothiocyanalte)或其它荧光色素。 3. PBS、DMSO 4. PH9~9.5碳酸盐缓冲
FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光
中英文品名 品牌 货号 规格 详情 DyLight 488 AffiniPure Goat Anti-Mouse IgG(H+L) EarthOx E032210 ¥200/100μl ¥800/500μl 详情 DyLight 488
流式课堂 | 易被忽略的 CD4、CD8 共表达细胞——胸腺淋巴细胞
做过流式实验的同学,对 CD4、CD8 这两个指标都不会陌生。检测 CD4+ T 细胞和 CD8+ T 细胞分型,是流式中最常见的实验之一。 通常,我们会先检测 CD3,来圈总T淋巴细胞,再分别用 CD4 抗体和 CD8 抗体,来区分 CD4+ T 淋巴细胞(辅助或调节性T细胞)和 CD8+ T 淋巴细胞(细胞毒性 T 细胞)。 新生小鼠脾脏细胞 上图为新生小鼠脾脏细胞进行的 CD3-PE、CD4-FITC、CD8-APC 三色流式实验结果。从图中可以看出,在新生小鼠脾脏中,绝大
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