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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR
- 靶点:
Acetyl-Histone H3 (Lys23)
- 抗体英文名:
Acetyl-Histone H3 (Lys23) Recombinant monoclonal antibody
- 抗体名:
Acetyl-Histone H3 (Lys23) Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive IHC detected in | mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive Dot Blot detected in | peptide |
| Positive ChIP-qPCR detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82838-2-RR targets Acetyl-Histone H3 (Lys23) in IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | Acetyl Histone, H3 Ac Lys23, H3 Acetyl K23, H3 Acetyl Lys23, H3 Acetyl Lysine23 |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_3670572 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白;在细胞正常 pH 值时,组蛋白带有正电荷,这样它们就可以和 DNA 结合,这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实,组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg , Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白: H1 , H2A , H2B , H3
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INTRODUCTION In cells and tissues, the histone proteins that constitute the nucleosomes can present multiple post-translational modifications, such as lysine acetylation, lysine and arginine methylation, serine phosphorylation
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