- ￥5800
- Proteintech
- 67616-1-PBS
- 2025年10月09日
- WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
- Mouse
- human, mouse, rat, pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human FREQ protein
- 亚型：
IgG2a
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
- 靶点：
NCS1
- 抗体英文名：
NCS1 Monoclonal antibody, PBS Only (Capture)
- 抗体名：
NCS1 Monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
67616-1-PBS targets NCS1 as part of a matched antibody pair:
MP51282-1: 67616-1-PBS capture and 67616-2-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, IF-P, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag29808 Product name: Recombinant human FREQ protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-190 aa of BC004856 Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | frequenin homolog (Drosophila) |
| 别名 | FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila) |
| 计算分子量 | 22 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC004856 |
| 基因名称 | NCS1 |
| Gene ID (NCBI) | 23413 |
| RRID | AB_2882819 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62166 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞，只有少数是分泌预定特异性单克隆抗体的细胞，因此，必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法，筛选出能产生所需单克隆抗体的阳性杂交瘤细胞，并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后，及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
Actin Capture Assay David Amberg Dialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 . Mix 5ug actin into 50ul total volume binding buffer. Mix 5ug GST-fusion protein into total volume 50ul binding
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