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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
Cytometric bead array, Indirect ELISA, Sample test
- 靶点:
Nectin-2/CD112
- 抗体英文名:
Mouse Nectin-2/CD112 Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名:
Mouse Nectin-2/CD112 Recombinant monoclonal antibody, PBS Only (Capture)
- 规格:
100ug
产品信息
84439-5-PBS targets Nectin-2/CD112 as part of a matched antibody pair:
MP01292-3: 84439-5-PBS capture and 84439-4-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA, Sample test |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2019 Product name: Recombinant Mouse Nectin-2/CD112 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 32-351 aa of NM_008990.3 Sequence: QDVRVRVLPEVRGRLGGTVELPCHLLPPTTERVSQVTWQRLDGTVVAAFHPSFGVDFPNSQFSKDRLSFVRARPETNADLRDATLAFRGLRVEDEGNYTCEFATFPNGTRRGVTWLRVIAQPENHAEAQEVTIGPQSVAVARCVSTGGRPPARITWISSLGGEAKDTQEPGIQAGTVTIISRYSLVPVGRADGVKVTCRVEHESFEEPILLPVTLSVRYPPEVSISGYDDNWYLGRSEAILTCDVRSNPEPTDYDWSTTSGVFPASAVAQGSQLLVHSVDRMVNTTFICTATNAVGTGRAEQVILVRESPSTAGAGATGG |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | poliovirus receptor-related 2 |
| 别名 | CD112, Herpes virus entry mediator B, HveB, mHveB, Mph |
| 计算分子量 | 57 kDa |
| GenBank蛋白编号 | NM_008990.3 |
| 基因名称 | Nectin-2/CD112 |
| Gene ID (NCBI) | 19294 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P32507-1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验.16 112.05 Dynabeads M-280 Streptavidin Supplied at 6.7x108 beads per ml (10mg/ml) in PBS, pH7.4 with 0.1% BSA and 0.02% sodium azide. Isolation of any biotinylated target. Applications include DNA, RNA and protein isolation
因为抗体的结合而减少;在人体血清中无游离的CD20存在;最重要的是B淋巴细胞瘤上的CD20少有内吞和脱落的发生[ 2-5 ] 。1997年,以CD20为靶点的嵌合抗CD20单克隆抗体Rituxan已获准应用于B细胞淋巴瘤的治疗[ 6 ]。HI47(IgG3)是我所于1990年研制成功的国内第一个抗CD20鼠源性单克窿抗体,第四届国际人类白细胞分化抗原会议将HI47命名为CD20+X[ 7 ] 。我们利用噬菌体显示技术从HI47杂交瘤细胞中克隆到抗CD20抗体HI47可变区基因,将该基因构建成为嵌合的抗
的,但是这结果是不是假阳性呢?还得做个阴性对照。阴性对照如何设置呢?考虑到整个实验体系会出现 beads、抗X抗体、诱饵蛋白 X、靶蛋白 Y(co-IP): 可能出现的假阳性来源 需要的阴性对照 抗 X 抗体结合非特异的蛋白 与 IP 一抗种属亚型相同的免疫球蛋白(比如,抗 X 抗体是 Mouse IgG,阴性对照可使用 Mouse 免疫球蛋白) beads 对蛋白的非特异结合* 同上,此外还可进行 Pre clear,IP 正式实验前排除非特异结合蛋白。如果使用琼脂
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