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- 详细信息
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
APOB
- 抗体英文名:
HRP-conjugated APOB Polyclonal antibody
- 抗体名:
HRP-conjugated APOB Polyclonal antibody
- 规格:
100ul
经过测试的应用
| Positive WB detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-20578 targets APOB in WB applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB |
| 经测试反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | apolipoprotein B (including Ag(x) antigen) |
| 别名 | Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB |
| 计算分子量 | 516 kDa |
| 观测分子量 | 150-250 kDa, 400-520 kDa |
| GenBank蛋白编号 | NM_000384 |
| 基因名称 | APOB |
| Gene ID (NCBI) | 338 |
| RRID | AB_2924163 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04114 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 and APOB-27.6 (PMID:11839763, PMID:2450346).
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