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- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite® Plus 405 Fluorescent Dye
- 宿主:
Rat
- 应用范围:
FC
- 靶点:
IgG1 Isotype Control
- 抗体英文名:
CoraLite® Plus 405 Rat IgG1 Isotype Control (HRPN)
- 抗体名:
CoraLite® Plus 405 Rat IgG1 Isotype Control (HRPN)
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥610.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1520.0 |
经过测试的应用
| Positive FC detected in |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65212 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.
| 经测试应用 | FC |
| 经测试反应性 | |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IgG1 Isotype Control |
| 别名 | IgG1, IgG1 Isotype Control, Rat IgG1 Isotype Control |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3083847 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The HRPN immunoglobulin is useful as an isotype-matched control. The HRPN immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.
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文献和实验SNFA-562 PE Rat IgG2a,κ Isotype Control(2A3) Rat PE SNFA-129 APC Rat IgG2a,κ Isotype Control(2A3) Rat APC SNFA-401 FITC Rat IgG1,κ Isotype Control(HRPN) Rat FITC SNFA-561 PE Rat IgG1,κ Isotype Control(HRPN) Rat PE
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
of antibody or control (normal rabbit serum, mouse isotype control,, etc.) to each tube and incubate overnight for 6 h at room temperature. 7) Add 100 µl of Protein-A-Sepharose solution (shake to suspend slurry before pipetting) and incubate on a tube
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