- ￥710 - 1780
- Proteintech
- CL405-65208
- 2025年10月09日
- FC
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 405 Fluorescent Dye
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
IgG2a Isotype Control
- 抗体英文名：
CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4)
- 抗体名：
CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥710.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1780.0 |
经过测试的应用
| Positive FC detected in |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65208 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.
| 经测试应用 | FC |
| 经测试反应性 | |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IgG2a Isotype Control |
| 别名 | IgG2a, IgG2a Isotype Control, Mouse IgG2a Isotype Control |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3083844 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.
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文献和实验(MOPC-21) Mouse APC SNFA-685 PerCP Mouse IgG1,κ Isotype Control(MOPC-21) Mouse PerCP SNFA-399 FITC Mouse IgG2a,κ Isotype Control(C1.18.4) Mouse FITC SNFA-559 PE Mouse IgG2a,κ Isotype Control(C1.18.4) Mouse PE SNFA
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
of antibody or control (normal rabbit serum, mouse isotype control,, etc.) to each tube and incubate overnight for 6 h at room temperature. 7) Add 100 µl of Protein-A-Sepharose solution (shake to suspend slurry before pipetting) and incubate on a tube
as many as 6 million dpm to maximize sensitivity. 4) Put the 100,000 dpm samples aside until step #12. 5) After lyophilization, solubilize 200,000 dpm samples in 350 µl IMP buffer. 6) Add 50 µl of antibody or control (normal rabbit serum, mouse isotype control
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