- ￥1350 - 3000
- Proteintech
- 68270-1-Ig
- 2025年10月09日
- WB, IHC, IF/ICC, ELISA
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human RPS20 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
RPS20
- 抗体英文名：
RPS20 Monoclonal antibody
- 抗体名：
RPS20 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68270-1-Ig targets RPS20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag8275 Product name: Recombinant human RPS20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-119 aa of BC007507 Sequence: MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S20 |
| 别名 | 3E9B4, 40S ribosomal protein S20, ribosomal protein S20, Small ribosomal subunit protein uS10 |
| 计算分子量 | 13 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC007507 |
| 基因名称 | RPS20 |
| Gene ID (NCBI) | 6224 |
| RRID | AB_2935353 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P60866 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IF | Nat Cell Biol Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificityAuthors - Joanna R Kovalski |
| human | WB | Sci Rep Hydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitisAuthors - Xuan He |
| human | IF | bioRxiv Functional screen for mediators of onco-mRNA translation specificityAuthors - Joanna R Kovalski |
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