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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
IF/ICC
- 靶点:
Cytokeratin 17-specific
- 抗体英文名:
CoraLite® Plus 488-conjugated Cytokeratin 17-specific Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 488-conjugated Cytokeratin 17-specific Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF/ICC detected in | HaCaT cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84978 targets Cytokeratin 17-specific in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | keratin 17 |
| 别名 | Cytokeratin 17, KRT17, 242240A8, CK 17, CK-17 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | NM_000422 |
| 基因名称 | Cytokeratin 17 |
| Gene ID (NCBI) | 3872 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q04695 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.The molecular weight of cytokeratin 17 is approximately 49 kDa.Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).
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文献和实验化钙,(问题所在),吹打均匀,孵育1~10min,测定荧光即Fmax值。 (4)加入EGTA,20μl(钙螯合剂),孵育1~10min,激发波长488nm测定荧光,即Fmin值。 这个过程中的氯化钙的作用如何, 请高人指点, 谢谢。 因为正常血浆中Ca2+浓度是1mM,细胞外液的Ca2+对细胞内Ca2+有影响。加0.1%triton是增加膜通透性,使细胞外Ca2+进入细胞内,作为最大值。加EGTA是为了螯合Ca2+,作为最小值.生理环境中细胞内钙离子浓度远低于细胞外液(大约1:10000
1~10min,测定荧光即Fmax值。(4)加入EGTA,20%26mu;l(钙螯合剂),孵育1~10min,激发波长488nm测定荧光,即Fmin值。这个过程中的氯化钙的作用如何, 请高人指点, 谢谢。因为正常血浆中Ca2+浓度是1mM,细胞外液的Ca2+对细胞内Ca2+有影响。加0.1%triton是增加膜通透性,使细胞外Ca2+进入细胞内,作为最大值。加EGTA是为了螯合Ca2+,作为最小值.生理环境中细胞内钙离子浓度远低于细胞外液(大约1:10000),细胞膜对钙的通透性变化对细胞内钙影响很大
1 mmol/L氯化钙,(问题所在),吹打均匀,孵育1~10min,测定荧光即Fmax值。 (4)加入EGTA,20%26mu;l(钙螯合剂),孵育1~10min,激发波长488nm测定荧光,即Fmin值。 这个过程中的氯化钙的作用如何, 请高人指点, 谢谢。 因为正常血浆中Ca2+浓度是1mM,细胞外液的Ca2+对细胞内Ca2+有影响。加0.1%triton是增加膜通透性,使细胞外Ca2+进入细胞内,作为最大值。加EGTA是为了螯合Ca2+,作为最小值.生理环境中细胞内钙离子浓度远低于
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