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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 靶点:
Cathepsin D
- 抗体英文名:
Cathepsin D Recombinant monoclonal antibody
- 抗体名:
Cathepsin D Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | Neuro-2a cells, RAW 264.7 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85964-1-RR targets Cathepsin D in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2986 Product name: Recombinant Mouse Cathepsin D protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 21-410 aa of NM_009983.3 Sequence: IIRIPLRKFTSIRRTMTEVGGSVEDLILKGPITKYSMQSSPKTTEPVSELLKNYLDAQYYGDIGIGTPPQCFTVVFDTGSSNLWVPSIHCKILDIACWVHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCKSDQSKARGIKVEKQIFGEATKQPGIVFVAAKFDGILGMGYPHISVNNVLPVFDNLMQQKLVDKNIFSFYLNRDPEGQPGGELMLGGTDSKYYHGELSYLNVTRKAYWQVHMDQLEVGNELTLCKGGCEAIVDTGTSLLVGPVEEVKELQKAIGAVPLIQGEYMIPCEKVSSLPTVYLKLGGKNYELHPDKYILKVSQGGKTICLSGFMGMDIPPPSGPLWILGDVFIGSYYTVFDRDNNRVGFANAVVL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cathepsin D |
| 别名 | Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD, CTSD |
| 计算分子量 | 45 kDa |
| 观测分子量 | 46 kDa, 28 kDa |
| GenBank蛋白编号 | NM_009983.3 |
| 基因名称 | Ctsd |
| Gene ID (NCBI) | 13033 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P18242 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTSD (Cathepsin D) also named CPSD, is a representative aspartic proteinase in lysosomes and is widely distributed in various tissue cells of mammals. The expression level of CTSD varies depending on the tissue, whereas neurons in CNS tissues possess abundant CTSD. CTSD is synthesized as a 52-kDa precursor that is converted into an active ~48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 28-kDa heavy chain and a 14-kDa light chain, in the lysosomes (PMID: 10995834, PMID: 7641679, PMID: 30051532).
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文献和实验Enzyme Analysis: Cathepsin D as an Example
In enzyme analysis, capillary electrophoresis (CE) offers the ease of product separation from the substrate, with the ability to use expensive reagents in microvolumes. In CE, enzymes can be measured either as mass (when they are present
组织蛋白酶 cathepsin 在各种动物组织的细胞内(特别是溶酶体部分)发现的一类蛋白酶。此名源自希腊语的“消化”,为威尔斯达特( R. Willstā atter, 1929)命名的。来源于同一种类组织细胞的也是由组织蛋白酶 A. B. C. D. E等多种酶组成。可以根据所作用的代表性底物的种类进行分类,但似乎各自还有数种同工酶。组织蛋白酶 A的代表性底物是 N-苄氧基羰基 -L-谷酰胺 -L-苯丙氨酸,最适 pH约 5.5( 3— 6),可能是一种羧肽酶,可为二异丙基磷氟酸
的关系图如下: 当发病率1时,OR高估了RR,当OR 设疾病在非暴露人群中的发病为 P0,则可用下列公式对 RR 记性校正: RR = OR/((1-P0)+(P0*OR)) 若P0未知,可以用c/(c+d)估计。 说到这里,可能就会有人疑惑了,OR与RR的区别到底在哪里?好像没什么区别。 继续往下看 在病例对照研究中,由于不能计算发病率,亦即不能计算发病率比(RR)。暴露和疾病联系强度就用比值比(OR)来近似表示RR
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