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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CAMP protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 靶点:
CAMP
- 抗体英文名:
CAMP Recombinant monoclonal antibody
- 抗体名:
CAMP Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | human saliva, mouse lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82940-1-RR targets CAMP in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag2622 Product name: Recombinant human CAMP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC055089 Sequence: MKTQRDGHSLGRWSLVLLLLGLVMPLAIIAQVLSYKEAVLRAIDGINQRSSDANLYRLLDLDPRPTMDGDPDTPKPVSFTVKETVCPRTTQQSPEDCDFKKDGLVKRCMGTVTLNQARGSFDISCDKDNKRFALLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cathelicidin antimicrobial peptide |
| 别名 | CAP18, 230157B4, Antibacterial peptide FALL-39, Antibacterial peptide FF-33, Antibacterial peptide KR-20 |
| 计算分子量 | 170 aa, 19 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC055089 |
| 基因名称 | CAMP |
| Gene ID (NCBI) | 820 |
| RRID | AB_3670682 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49913 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.
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文献和实验| Species | Application | Title |
|---|---|---|
| rat | WB | J Ethnopharmacol Study on the underlying mechanism of Huachansu Capsule induced cardiotoxicity of normal rat by integrating transcriptomics, metabolomics and network toxicologyAuthors - Qiang-Qiang Fan |
多克隆抗体制备方法有必然关系,如果不乙酰化将无法达到检测所需的灵敏度。 所以无论标记方法如何革新,不改进抗体,仍然不能从根本上迎来cAMP,cGMP定量检测的变革。 cAMP和cGMP单克隆抗体成功开发 NewEast Biosciences(纽斯特)公司的科学家经过两年的研发,筛选了4万多个克隆,终于从中发现了高特异性,高亲和力的cAMP和cGMP单克隆抗体。并成功构建了世界上唯一的基于单抗的cAMP和cGMP ELISA
,这种方法能不能用来检测脑组织cAMP的含量呢? wlily 同问,我最近用R&D的试剂盒测,做三次,两次标准曲线很好,但样品的cAMP浓度不稳定 涛哥来了 我也是用的R &D公司 为什么我的每次都检测不到啊,我试过超声波和细胞裂解液(同时加了PDE抑制剂)处理细胞 然后离心取上清。能请教下你是怎么做的么? xwbcn 建议用NewEast Biosciences公司的基于单克隆抗体的cAMP
,cGMP定量检测的变革。 cAMP和cGMP单克隆抗体成功开发 NewEast Biosciences(纽斯特)公司的科学家经过两年的研发,筛选了4万多个克隆,终于从中发现了高特异性,高亲和力的cAMP和cGMP单克隆抗体。并成功构建了世界上唯一的基于单抗的cAMP和cGMP ELISA检测试剂盒。这个cAMP(cGMP)单抗对非乙酰化的cAMP(cGMP)的特异性识别能力是其对cGMP(cAMP)、ATP(GTP)以及其他核苷类似物识别能力的108倍以上,这相比
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