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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CSNK2A1 protein
- 亚型:
IgG1
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human, rabbit
- 宿主:
Mouse
- 应用范围:
WB, IF/ICC, Indirect ELISA
- 靶点:
CSNK2A1
- 抗体英文名:
CSNK2A1 Monoclonal antibody, PBS Only
- 抗体名:
CSNK2A1 Monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
68200-1-PBS targets CSNK2A1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rabbit samples.
| 经测试应用 | WB, IF/ICC, Indirect ELISA |
| 经测试反应性 | human, rabbit |
| 免疫原 |
CatNo: Ag29062 Product name: Recombinant human CSNK2A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-391 aa of BC011668 Sequence: MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | casein kinase 2, alpha 1 polypeptide |
| 别名 | 1D5E8, CK II alpha, CK2a, CK2A1, CK2α |
| 计算分子量 | 45 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC011668 |
| 基因名称 | CSNK2A1 |
| Gene ID (NCBI) | 1457 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P68400 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
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文献和实验1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞,只有少数是分泌预定特异性单克隆抗体的细胞,因此,必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法,筛选出能产生所需单克隆抗体的阳性杂交瘤细胞,并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后,及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
过夜。(5)用少许PBS将交联物全部洗出,收集洗出液,加1/20V体积新鲜配制的5mg/ml NaBH4溶液,混匀,室温作用30分钟;再加入3/20V NaBH4溶液,混匀,室温作用1小时(或4℃过夜)。(6)将交联物过Sephadex G200或Sepharose 6B(2.6×50cm)层析纯化,分管收集第一峰。(7)酶结合物质量鉴定:克分子比值测定酶量(mg/ml)=OD403×0.4IgG量(mg/ml)=(OD280-OD403×0.3)×0.62克分子比值(E/P)=酶量×4/IgG量
一、材料及试剂 1、猪瘟单抗纯化抗原 2、兔抗猪IgG酶标抗体 3、猪瘟阳性血清 4、猪瘟阴性血清 1~4均由指定的生物制品所购买。 5、ELISA反应板 6、包被液 碳酸钠 1.50g 碳酸氢钠 2.93g H2O加至 1 000ml pH值9.6 7、PBS液 氯化钠 8.90g 磷酸二氢钾 0.20g 无水磷酸氢二钠 2.13g 加双馏水 1 000ml 8、PBS-吐温洗涤液 PBS液 1 000ml 犊牛血清 5ml 混合即可 9、底物
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