- ￥2800
- Proteintech
- CL555-60183
- 2026年06月05日
- IF/ICC, IF-P
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KRT20 protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®555 Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
IF/ICC, IF-P
- 靶点：
Cytokeratin 20
- 抗体英文名：
CoraLite®555-conjugated Cytokeratin 20 Monoclonal antibody
- 抗体名：
CoraLite®555-conjugated Cytokeratin 20 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | human colon tissue |
| Positive IF/ICC detected in | HT-29 cells, human colon cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-60183 targets Cytokeratin 20 in IF/ICC, IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, IF-P |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag11336 Product name: Recombinant human KRT20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 74-424 aa of BC031559 Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | keratin 20 |
| 别名 | KRT20, keratin 20, K20, Cytokeratin-20, CK-20 |
| 计算分子量 | 424 aa, 48 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC031559 |
| 基因名称 | Cytokeratin 20 |
| Gene ID (NCBI) | 54474 |
| RRID | AB_2923893 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35900 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
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