- ￥1350 - 3000
- Proteintech
- 60875-5-Ig
- 2025年10月09日
- WB, IHC, IF/ICC, ELISA
- Mouse
- human, rat, rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human FTH1 protein
- 亚型：
IgG2a
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, rat, rabbit
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
Ferritin heavy chain
- 抗体英文名：
Ferritin heavy chain Monoclonal antibody
- 抗体名：
Ferritin heavy chain Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A549 cells, rabbit brain tissue, HeLa cells, K-562 cells, rat brain tissue |
| Positive IHC detected in | rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60875-5-Ig targets Ferritin heavy chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, rabbit samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 经测试反应性 | human, rat, rabbit |
| 免疫原 |
CatNo: Ag21386 Product name: Recombinant human FTH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-183 aa of BC000857 Sequence: MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ferritin, heavy polypeptide 1 |
| 别名 | Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed |
| 计算分子量 | 183 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC000857 |
| 基因名称 | FTH1 |
| Gene ID (NCBI) | 2495 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P02794 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.
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文献和实验resolution. A detailed protocol of RT-TDPCR is presented here with examples of its use with ribonuclease T1 in mammalian cells for detecting (1) RNA structure and protein footprints of the human ferritin heavy-chain messenger RNA and (2) in vivo structure
)与lambda(λ)，同一个天然Ig分子上L链的型总是相同的。正常人血清中的κ：λ约为2：1。 2．重链（heavy chain,H链） 重链大小约为轻链的2倍，含450～550个氨基酸残基，分子量约为55或75kD。每条H链含有4～5个链内二硫键所组成的环肽。不同的H链由于氨基酸组成的排列顺序、二硫键的数目和们置、含的种类和数量不同，其抗原性也不相同，根据H链抗原性的差异可将其分为5类：μ链、γ链、α链、δ链和ε链，不同H链与L链（κ或λ链）组成完整Ig的分子分别称之为IgM、IgG、IgA
有很少的病人[2-5]对移植的器官或组织不发生排斥,但这又往往表示由于抑制药物的作用,这些病例通常都是十分珍贵的。由于对人和啮齿类动物免疫系统之间的差别知之甚少,何况比起啮齿类动物,在人的身上诱导免疫耐受也存在着明显的困难.所以在器官移植的实验方案中,主要选用个体较大的动物作为实验对象,猪和灵长类动物的基因与人类基因有着极为相似性.尤其是恒河猴同人之间的MHCⅠⅡ分子结构有着同源相似性[6,7].不过这只是从理论上而讲的,灵长类动物和人之间的免疫抑制机制的准确,详细的联系我们还不清楚也几乎没有
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