- ￥5800
- Proteintech
- 60875-5-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, Indirect ELISA
- Mouse
- human, rat, rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human FTH1 protein
- 亚型：
IgG2a
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, rat, rabbit
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, Indirect ELISA
- 靶点：
Ferritin heavy chain
- 抗体英文名：
Ferritin heavy chain Monoclonal antibody, PBS Only
- 抗体名：
Ferritin heavy chain Monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
60875-5-PBS targets Ferritin heavy chain in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, rabbit samples.
| 经测试应用 | WB, IHC, IF/ICC, Indirect ELISA |
| 经测试反应性 | human, rat, rabbit |
| 免疫原 |
CatNo: Ag21386 Product name: Recombinant human FTH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-183 aa of BC000857 Sequence: MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ferritin, heavy polypeptide 1 |
| 别名 | Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed |
| 计算分子量 | 183 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC000857 |
| 基因名称 | FTH1 |
| Gene ID (NCBI) | 2495 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P02794 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
minute washes in PBS. 7) Add 5% donkey serum in PBS to the samples for 1 ½ hours to block any non-specific binding of the secondary antibody to the samples. 8) Add Connexin 43 (1:50 dilution) and Myosin Heavy Chain (1:100
核悬液1/2量的0.1n HCl , 0.5% Triton X-100。室温停留10min。加入IBM-0.25%Triton X-100（IBM配方：50mmol/l KCl, 10mmol/L MgSO4 , 5mmol/L HEPES pH8.0）。再离心，重复IBM漂洗（这时细胞核可在不染色情况下，以荧光显微镜观察）后，以2×SSC-0.1%Tween漂洗1×3min，继之加入等量2%的多聚甲醛在1×PBS-5mmol/l MgSO4 。在室温静置站立10min。倾去上清液，加IBm
，仍应结合碘液涂片法以检出原虫包囊。 7.虫卵计数法 虫卵计数用于估计人体内寄生虫的感染度，常用司徒尔(Stoll)氏法，即司氏稀释虫卵计数法(图22-5)。 图22-5 司氏虫卵计数法 图22-6 定量板 用特制的三角烧瓶(或普通三角烧瓶)，容量为65ml左右，在烧瓶的颈部相当于56和60ml处有两个刻度。先把0.1mol/L NaOH溶液倒入瓶内至56ml处，再慢慢地加入粪便，到液面上升到60ml处，然后放
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