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- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
mouse
- 宿主:
Rabbit
- 应用范围:
FC (Intra)
- 靶点:
IL-15
- 抗体英文名:
CoraLite® Plus 488 Anti-Mouse IL-15 Rabbit Recombinant Antibody
- 抗体名:
CoraLite® Plus 488 Anti-Mouse IL-15 Rabbit Recombinant Antibody
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1200.0 |
经过测试的应用
| Positive FC (Intra) detected in | mouse PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.5 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-98036 targets IL-15 in FC (Intra) applications and shows reactivity with mouse samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg0359 Product name: recombinant mouse il15 protein Source: mammalian cells-derived, pHZ-KIsec-Cfc-2 Tag: C-FC Domain: 49-162 aa of NM_001254747 Sequence: NWIDVRYDLEKIESLIQSIHIDTTLYTDSDFHPSCKVTAMNCFLLELQVILHEYSNMTLNETVRNVLYLANSTLSSNKNVAESGCKECEELEEKTFTEFLQSFIRIVQMFINTS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 15 |
| 别名 | 230285B3, AI503618, Il15, interleukin 15 |
| 计算分子量 | 19 kDa |
| GenBank蛋白编号 | NM_001254747 |
| 基因名称 | IL-15 |
| Gene ID (NCBI) | 16168 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P48346 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
IL-15 is a 4-α-helix bundle cytokine playing a pivotal role in stimulation of both innate and adaptive immune cells. It is produced primarily by keratinocytes, skeletal muscle cells, monocytes, and CD4+ T cells. It is a member of the common gamma chain family. The members of the common gamma chain family include the IL-2, IL-4, IL-7, IL-9, and IL-21 and require binding to the common gamma chain receptor for activation. It can be used in growth and maintenance of T and NK cells. It is also shown to be used in proliferation and functional effect of T cells for adoptive cell therapy. It is a glycosylated protein and HumanKine IL-15 appear as 12.5-25 kDa bands (PMID: 24587813, 26627006, 27849617, 31250350)
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文献和实验secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
1R1 Mammalian Cells 48-66 kDa P4851 Recombinant Human IL-1RA E.Coli 19 kDa Pr3583 Recombinant Mouse IL-1b E.Coli 16 kDa P4424 Recombinant Mouse IL-1A E.Coli 17.1 kDa Pr22491 Recombinant Mouse IL-
[欢迎下载]:Antibody Engineering: Methods and Protocols
. coli 15 Expression of Recombinant Antibodies in Mammalian Cell Lines 16 Human Antibody Production Using Insect-Cell Expression Systems 17 Antibody Production in Transgenic Plants 18 Directed Mutagenesis of Antibody Variable Domains 19 Antibody
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