- ￥1350 - 3000
- Proteintech
- 68197-1-Ig
- 2025年10月09日
- WB, IF/ICC, ELISA
- Mouse
- Human, Rabbit
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human VPS25 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Rabbit
- 宿主：
Mouse
- 应用范围：
WB, IF/ICC, ELISA
- 靶点：
VPS25
- 抗体英文名：
VPS25 Monoclonal antibody
- 抗体名：
VPS25 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, rabbit kidney tissue, HepG2 cells, Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68197-1-Ig targets VPS25 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA |
| 经测试反应性 | Human, Rabbit |
| 免疫原 |
CatNo: Ag8243 Product name: Recombinant human VPS25 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-176 aa of BC006282 Sequence: MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 25 homolog (S. cerevisiae) |
| 别名 | DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25 |
| 计算分子量 | 176 aa, 21 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC006282 |
| 基因名称 | VPS25 |
| Gene ID (NCBI) | 84313 |
| RRID | AB_2935286 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9BRG1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).
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