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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR
- 靶点:
Acetyl-Histone H4 (Lys12)
- 抗体英文名:
Acetyl-Histone H4 (Lys12) Recombinant monoclonal antibody, PBS Only
- 抗体名:
Acetyl-Histone H4 (Lys12) Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
83095-1-PBS targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H4a |
| 别名 | Ac Histone H4, Ac Histone H4 (Lys12), Ac Histone H4 Lys12, Ac Histone H4-Lys12, Acetyl Histone |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC069654 |
| 基因名称 | HIST1H4A |
| Gene ID (NCBI) | 8359 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62805 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.
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文献和实验消化PC12是个错误观点,其一针对悬浮PC12,胰酶消化的效果不好证实,其二,我养的PC12,性质贴壁,用100%胰酶消化1小时,愣是没动静,细胞甚至不变圆。最后用力拍打才可以,但代价是细胞损伤非常大,不好恢复。其三,ATCC上明确建议用细胞刮勺刮取PC12,目前我改用刮勺,细胞传代后状态要比用胰酶消化的好很多。 4.再者关于1640和DMEM的问题,我个人观点,二者的主要区别在于DMEM含糖量高,神经细胞都喜欢这,另外1640含一些离子,是防止培养的细胞贴壁。1640一开始主要应用于单克隆抗体
is lost on tips). Rotate in cold room for 1 hr. Spin and wash beads twice with RIPA (discard supernatant), and then once with Histone Wash Buffer. Add 30 µL of Histone Assay solution. Incubate at 37°C for exactly 30 min. Mix
液,加入6ml DMEM完全培养基,37℃孵育过夜,再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变(CPE)出现(如用pAdTrack-CMV质粒,由于含GFP,可观察到绿色荧光)。四、重组病毒鉴定 1、转导10-14d后,收集细胞沉淀,加入2ml灭菌的PBS混悬,冻融细胞,离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清,感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d,当1/3-1/2细胞漂浮时
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