FcZero-rAb™ CoraLite® Plus 488
Anti-Mouse CD86 Rabbit Recombinant Antibody CL488-FcA98025- ￥750 - 1500
- Proteintech
- CL488-FcA98025
- 2025年10月09日
- FC
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
CD86
- 抗体英文名：
FcZero-rAb™ CoraLite® Plus 488 Anti-Mouse CD86 Rabbit Recombinant Antibody
- 抗体名：
FcZero-rAb™ CoraLite® Plus 488 Anti-Mouse CD86 Rabbit Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥750.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1500.0 |
经过测试的应用
| Positive FC detected in | LPS treated mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-FcA98025 targets CD86 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg0802 Product name: Recombinant Mouse CD86 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 24-245 aa of NM_019388 Sequence: VSVETQAYFNGTAYLPCPFTKAQNISLSELVVFWQDQQKLVLYEHYLGTEKLDSVNAKYLGRTSFDRNNWTLRLHNVQIKDMGSYDCFIQKKPPTGSIILQQTLTELSVIANFSEPEIKLAQNVTGNSGINLTCTSKQGHPKPKKMYFLITNSTNEYGDNMQISQDNVTELFSISNSLSLSFPDGVWHMTVVCVLETESMKISSKPLNFTQEFPSPQTYWKE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD86 antigen |
| 别名 | 230476B8, B7, B7 2, B7.2, B70 |
| 计算分子量 | 35 kDa |
| GenBank蛋白编号 | NM_019388 |
| 基因名称 | Cd86 |
| Gene ID (NCBI) | 12524 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P42082-1 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
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文献和实验secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
Technovit® 9100 Methyl Methacrylate
with buffer (see above) DAKO EnVision polyvalent antibody (goat-anti-mouse/goat-anti-rabbit) coupled to alkaline phosphatase 30 min at room temperature Visualization Step
overnight. Next, slides were rinsed with water and covered with blocking solution for 2 h at room temperature. Then, after rinsing with water, AlexaFluor-488 labeled anti-mouse antibody conjugate (33 nM in blocking solution) was added to the sample slides
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