- ￥5800
- Proteintech
- 85975-5-PBS
- 2025年10月09日
- Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
CD85j / LILRB1
- 抗体英文名：
CD85j / LILRB1 Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
CD85j / LILRB1 Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
85975-5-PBS targets CD85j / LILRB1 as part of a matched antibody pair:
MP02236-2: 85975-5-PBS capture and 85975-4-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg3701 Product name: Recombinant Human LILRB1/CD85j protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 24-458 aa of BC015731 Sequence: GHLPKPTLWAEPGSVITQGSPVTLRCQGGQETQEYRLYREKKTAPWITRIPQELVKKGQFPIPSITWEHAGRYRCYYGSDTAGRSESSDPLELVVTGAYIKPTLSAQPSPVVNSGGNVTLQCDSQVAFDGFILCKEGEDEHPQCLNSQPHARGSSRAIFSVGPVSPSRRWWYRCYAYDSNSPYEWSLPSDLLELLVLGVSKKPSLSVQPGPIVAPEETLTLQCGSDAGYNRFVLYKDGERDFLQLAGAQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSEWSAPSDPLDILIAGQFYDRVSLSVQPGPTVASGENVTLLCQSQGWMQTFLLTKEGAADDPWRLRSTYQSQKYQAEFPMGPVTSAHAGTYRCYGSQSSKPYLLTHPSDPLELVVSGPSGGPSSPTTGPTSTSGPEDQPLTPTGSDPQSGLGRH |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 |
| 别名 | LILRB1, CD85, CD85 antigen-like family member J, CD85j, FLJ37515 |
| 计算分子量 | 71 kDa |
| GenBank蛋白编号 | BC015731 |
| 基因名称 | LILRB1 |
| Gene ID (NCBI) | 10859 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8NHL6 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Immuno-Laser Capture Microdissection
with the primary and secondary antibodies and the tertiary reagent for 90 to 120 seconds each, rinsing briefly with 1 x PBS between each step. Develop the color with diaminobenzidine (DAB) for 3 to 5 minutes and counterstain with hematoxylin for 15 to 30
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
Bajno, L., Peng, X.R., Schreiber, A.D., Moore, H.P., Trimble, W.S., and Grinstein, S. 2000. Focal exocytosis of VAMP3‐containing vesicles at sites of phagosome formation. J. Cell Biol. 149:697‐706.
孵育1-2小时，洗涤，拍干。 h. 加底物液，每孔加新鲜配制的底物使用液50-100ul，37℃10-30分钟。 i. 以2mol/L H2SO4终止反应，在酶联免疫阅读仪上读取OD值。 j. 结果判定：以P/N≧2.1，或P≧N+3D为阳性。若阴性对照孔无色或接近无色，阳性对照孔明确显色，则可直接用肉眼观察结果。 C、 全菌抗原的ELISAS a. 新鲜培养的细菌用蒸馏水或PBS悬浮，并调整细菌浓度至1×108个/ml。必须指出
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