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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CDH15 protein
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, IF-P, Indirect ELISA
- 靶点:
M-cadherin
- 抗体英文名:
M-cadherin Recombinant monoclonal antibody, PBS Only
- 抗体名:
M-cadherin Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
84440-5-PBS targets M-cadherin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, Indirect ELISA |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25763 Product name: Recombinant human CDH15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-450 aa of BC008951 Sequence: AFLQEAFTGRVLEGAVPGTYVTRAEATDADDPETDNAALRFSILQQGSPELFSIDELTGEIRTVQVGLDREVVAVYNLTLQVADMSGDGLTATASAIITLDDINDNAPEFTRDEFFMEAIEAVSGVDVGRLEVEDRDLPGSPNWVARFTILEGDPDGQFTIRTDPKTNEGVLSIVKALDYESCEHYELKVSVQNEAPLQAAALRAERGQAKVRVHVQDTNEPPVFQENPLRTSLAEGAPPGTLVATFSARDPDTEQLQRLSYSKDYDPEDWLQVDAATGRIQTQHVLSPASPFLKGGWYR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cadherin 15, type 1, M-cadherin (myotubule) |
| 别名 | CDH15, 241837C10, Cadherin 14, Cadherin 15, Cadherin-14 |
| 计算分子量 | 89 kDa |
| 观测分子量 | 90-130 kDa |
| GenBank蛋白编号 | BC008951 |
| 基因名称 | M-cadherin |
| Gene ID (NCBI) | 1013 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P55291 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).
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文献和实验液,加入6ml DMEM完全培养基,37℃孵育过夜,再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变(CPE)出现(如用pAdTrack-CMV质粒,由于含GFP,可观察到绿色荧光)。四、重组病毒鉴定 1、转导10-14d后,收集细胞沉淀,加入2ml灭菌的PBS混悬,冻融细胞,离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清,感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d,当1/3-1/2细胞漂浮时
质沉淀,尤其是PI值在4.5附近的白蛋白,将这些杂蛋白用辛酸沉淀完毕后,再用法将抗体沉淀下来即可。 具体操作: (1)将腹水用0.06 M醋酸钠(pH4.0)按1:3稀释,在4 ℃条件下搅拌加入正辛酸,一般按每毫升未稀释的腹水加入40 ul正辛酸,然后4 ℃静置2 h; (2)4 ℃10000 g离心20min,可见辛酸因温度低而从系统中结晶析出漂浮在腹水上层,弃去此结晶以及底部沉淀,向上清中加入1/10体积的10×PBS(0.1M,pH 7.4),4 ℃条件下向其中加
-DNA液,加入6ml DMEM完全培养基,37℃孵育过夜,再换液。 7 培养过程中观察细胞生长情况。约2周后可观察到细胞病变(CPE)出现(如用pAdTrack-CMV质粒,由于含GFP,可观察到绿色荧光)。 四 重组病毒鉴定 1 转导10-14d后,收集细胞沉淀,加入2ml灭菌的PBS混悬,冻融细胞,离心后收集上清保存于-80℃。 2 取30-50% 步骤1中上清,感染50-70%饱和度的25cm2 方瓶中293细胞。2-3d后出现明显细胞
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