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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
mouse, rat
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
CD21/CD35
- 抗体英文名:
CD21/CD35 Recombinant monoclonal antibody
- 抗体名:
CD21/CD35 Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | A20 cells, mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84565-2-RR targets CD21/CD35 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 经测试反应性 | mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | complement receptor 2 |
| 别名 | CD21, Cr2, 241957E6 |
| 计算分子量 | 113kDa |
| 观测分子量 | 160-210 kDa |
| GenBank蛋白编号 | AAA37448.1 |
| 基因名称 | Cr2 |
| Gene ID (NCBI) | 12902 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P19070 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 short consensus repeats (SCRs) (PMID: 6230668; 2551147). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, binds C3b and C4b. The murine Cr2 gene encodes for both CD21 and CD35 (PMID: 2139460; 19559484). Mouse CD35 comprises 21 SCRs while mouse CD21 is identical to mouse CD35 except that it lacks the first 6 SCRs. CD21/CD35 forms trimolecular complexes with CD19 and CD81 and plays roles in the regulation of humoral immune responses (PMID: 11043778).
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文献和实验B 细胞内抗体产生新机制!两篇 Nature 重磅揭示染色质环挤压导致 V(D)J 重组和抗体类别转换重组
12F3 B 淋巴细胞作为实验对象(该细胞系只能由 IgM 转换成 IgA),敲除 Iα 后用 Anti-CD40-IL-4-TGFβ 作为诱导物,同样利用 GRO-seq,3C-HTGTS 和 ChIP-seq 等测序技术来评估 Iα 转录在 AID 之外的 SαCSR 中的潜在作用。研究人员发现,黏着蛋白介导的环挤压促进 3’RR 激活 IgH 恒定区上游转录单位和 Sm-Sx 配对,并揭示了 CH12F3 细胞只进行 IgA 重组的机制。最后,研究人员又继续探究了 CH12F3NCΔ 细胞
),CR2(CD21),CR3(CD11b/CD18),CR4(gp150,90:CD11c/CD18)。其主要特征列于表3-5。此外,尚有其他补体成分的受体,如Ciq受体、C3a受体、C5a受体等。因对其了解不够清楚,不拟介绍。 表3-5 补体受体的特征 名称 别名 CD分类 配体特异性 细胞分布 CR1 IA受体 C3b受体 C4B/C3b受体 CD35 C3bi、C3b C4b、iC4b C3c 红细胞、中性粒细胞 单核细胞、巨噬细胞
补体成分激活后产生的裂解片段,能与免疫细胞表面的特异性受体结合。这对于补体发挥其生物学活性具有重要意义。 补体受体(complementruceptor,CR)曾按其所结合配体而命名为C3b受体、C3d受体等;但经详细研究后发现,补体受体并非仅与C3裂解产物反应,因而按其发现先后依次命名为CR1(CD35),CR2(CD21),CR3(CD11b/CD18),CR4(gp150,90:CD11c/CD18)。其主要特征列于表3-5
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