- ￥5800
- Proteintech
- 85863-3-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
Cytometric bead array, Indirect ELISA
- 靶点：
M/R Sost
- 抗体英文名：
Mouse M/R Sost Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
Mouse M/R Sost Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
85863-3-PBS targets M/R Sost as part of a matched antibody pair:
MP02169-1: 85863-3-PBS capture and 85863-1-PBS detection (validated in Cytometric bead array)
MP02169-2: 85863-3-PBS capture and 85863-2-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg3294 Product name: Recombinant Mouse SOST/Sclerostin protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 24-211 aa of NM_024449.6 Sequence: QGWQAFRNDATEVIPGLGEYPEPPPENNQTMNRAENGGRPPHHPYDAKDVSEYSCRELHYTRFLTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRVKWWRPNGPDFRCIPDRYRAQRVQLLCPGGAAPRSRKVRLVASCKCKRLTRFHNQSELKDFGPETARPQKGRKPRPGARGAKANQAELENAY |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sclerostin |
| 别名 | Sclerostin, Sost, SOST/Sclerostin |
| 计算分子量 | 23KD |
| GenBank蛋白编号 | NM_024449.6 |
| 基因名称 | Sost |
| Gene ID (NCBI) | 74499 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99P68 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验+ 0.01% Triton X-100 + 1mM DTT + 1mM EGTA. Variables include lowering salt (above recipe is about 136mM in NaCl). replacing EGTA with 1m M CaCl2, replacing ATP with ADP. ATP Sigma#A5394 ADP Sigma #A6521 10X PBS: 80 grams NaCl + 2 grams
Immuno-Laser Capture Microdissection
with spot sizes of 50 to 60 µm (C) and 15 to 20 µm (D) in diameter, as seen during LCM without coverslip. The smaller spot size allows avoidance of interspersed T cells. Magnification x100 (C) and x400 (D). Fig. E: Lymph node stained
+ 0.1% BSA + 0.01% Triton X-100 + 1mM DTT + 1mM EGTA. Variables include lowering salt (above recipe is about 136mM in NaCl). replacing EGTA with 1m M CaCl2, replacing ATP with ADP. ATP Sigma#A5394 ADP Sigma #A6521 10X PBS: 80 grams NaCl
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