- ￥1350 - 3000
- Proteintech
- 15777-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HLA class I (HLA-C) protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
HLA class I (HLA-C)
- 抗体英文名：
HLA class I (HLA-C) Polyclonal antibody
- 抗体名：
HLA class I (HLA-C) Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15777-1-AP targets HLA class I (HLA-C) in WB, IHC, IF/ICC, IP, ELISA, Cell treatment applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, Cell treatment |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8461 Product name: Recombinant human HLA class I (HLA-C) protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-305 aa of BC007814 Sequence: SMRYFDTAVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQADRVSLRNLRGYYNQSEDGSHTLQRMSGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKLEAARAAEQLRAYLEGTCVEWLRRYLENGKETLQRAEPPKTHVTHHPLSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHMQHEGLQEPLTLSWEPSSQPTI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class I, C |
| 别名 | HLA-C, HLAC, HLA JY3, HLA Cw12, HLA Cw |
| 计算分子量 | 366 aa, 41 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | BC007814 |
| 基因名称 | HLA class I (HLA-C) |
| Gene ID (NCBI) | 3107 |
| RRID | AB_1639892 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10321 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250)
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | J Matern Fetal Neonatal Med HLA-C promotes proliferation and cell cycle progression in trophoblast cellsAuthors - Hong Lv |
| human | IHC | Front Pharmacol Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosisAuthors - Yanling Wang |
| human | Cell treatment | Nature Shifts in receptors during submergence of an encephalitic arbovirusAuthors - Wanyu Li |
| mouse | IF | Neuroscience HLA is a potent immunoinflammatory target in asymptomatic Alzheimer's diseaseAuthors - Yingwei Zheng |
Generation of HLA Class I Transfected Target Cell Lines
Normal human lymphoblastoid cell lines (LCLs) express six classical class I HLA genes from three gene loci, HLA-A, HLA-B, and HLA-C, which are located within the major histocompatibility complex (MHC). Because of the extensive allelic
Immunogenetics and Life-Span: HLA
-B, HLA-C) and HLA class II (HLA-DR, HLA-DQ, HLA-DP) cell surface glycoproteins present antigenic peptides to CD8+ and CD4+ T cells, respectively, and play a central role in mediating the immune response. The HLA class I and class II genes display extreme
PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles
Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) as a method for the definition of human leukocyte antigen (HLA ) alleles has most often been applied as a typing technique for HLA class II . The best
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