- ￥3200
- Proteintech
- CL488-67898
- 2026年05月28日
- IF/ICC, FC (Intra)
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Mouse
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
Phospho-S6 Ribosomal protein (Ser235)
- 抗体英文名：
CoraLite® Plus 488-conjugated Phospho-S6 Ribosomal protein (Ser235) Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated Phospho-S6 Ribosomal protein (Ser235) Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | Calyculin A treated HeLa cells |
| Positive FC (Intra) detected in | Calyculin A treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67898 targets Phospho-S6 Ribosomal protein (Ser235) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S6 |
| 别名 | p S6 Ribosomal protein Ser235, p S6 Ribosomal protein (Ser235), p S6 Ribosomal protein, Phospho S6 Ribosomal protein, Phospho S6 Ribosomal protein (Ser235) |
| 计算分子量 | 29 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC000524 |
| 基因名称 | RPS6 |
| Gene ID (NCBI) | 6194 |
| RRID | AB_2934584 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62753 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 67898-1-Ig specifically recognizes the phosphorylation site of Ser235 or dual site Ser235/236.
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文献和实验TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
ribosomal protein S5 (RPS5), mRNA 6739 NM_003021 Homo sapiens small glutamine-rich tetratricopeptide repeat (TPR)-containing (SGT), mRNA 488 NM_004285 Homo sapiens hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), mRNA 646 NM_004142
光物质，它在受到紫外线照射后可以发出一可见光，常用于DNA、RNA电泳中。 APC 英文全名：allophycocyanin；中文名：别藻青蛋白；最大吸收峰：650nm，最大发射荧光峰：660nm，适用于双激光流式仪，可被600-640nm波长的激光激发。 PerCP 英文全名：peridinin chlorophyll protein，中文名：多甲藻叶绿素蛋白；最大激发波峰490nm，被488nm的氩离子激光激发后，发射光峰值约为677nm，与FITC、PE进行多色荧光染色补偿重叠较少，非特异性结合
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