- ￥700 - 1750
- Proteintech
- CL750-65124
- 2025年10月09日
- FC
- Mouse
- n/a
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
n/a
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
IgG1 Isotype Control
- 抗体英文名：
CoraLite® Plus 750 Mouse IgG1 Isotype Control (MOPC-21)
- 抗体名：
CoraLite® Plus 750 Mouse IgG1 Isotype Control (MOPC-21)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥700.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1750.0 |
经过测试的应用
| Positive FC detected in |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC |
| 经测试反应性 | n/a |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant gamma 1 (G1m marker) |
| 别名 | Ighg1, MOPC-21, Igh-4, IgG1 |
| 基因名称 | IgG1 Isotype Control |
| Gene ID (NCBI) | 16017 |
| RRID | AB_3084856 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P01868 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.
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文献和实验as many as 6 million dpm to maximize sensitivity. 4) Put the 100,000 dpm samples aside until step #12. 5) After lyophilization, solubilize 200,000 dpm samples in 350 µl IMP buffer. 6) Add 50 µl of antibody or control (normal rabbit serum, mouse isotype control
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
of antibody or control (normal rabbit serum, mouse isotype control,, etc.) to each tube and incubate overnight for 6 h at room temperature. 7) Add 100 µl of Protein-A-Sepharose solution (shake to suspend slurry before pipetting) and incubate on a tube
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
input samples may need to be extracted twice.20. Add 30 uls of 5M NaCl, 5 ugs of tRNA and 5 ugs of glycogen to each sample. Mix well then add 750 uls of ethanol. Precipitate in -200 C freezer overnight.Day 4 21. Microfuge samples at 14,000 rpm for 20
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