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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
IHC, FC
- 靶点:
CD138/Syndecan-1
- 抗体英文名:
Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody, PBS Only
- 抗体名:
Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody, PBS Only
- 规格:
500ug/1mg
| 规格: | 500ug | 产品价格: | ¥3600.0 |
|---|---|---|---|
| 规格: | 1mg | 产品价格: | ¥5000.0 |
经过测试的应用
| Positive FC detected in | U266 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98051-1-PBS targets CD138/Syndecan-1 in IHC, FC applications and shows reactivity with human samples.
| 经测试应用 | IHC, FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | syndecan 1 |
| 别名 | CD138, 240422C4, syndecan, SYND1, SDC1 |
| 计算分子量 | 32.5 kDa |
| GenBank蛋白编号 | BC008765 |
| 基因名称 | CD138 |
| Gene ID (NCBI) | 6382 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P18827 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
suspended in PBS. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600
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