- ￥5800
- Proteintech
- 66030-1-PBS
- 2025年10月09日
- WB, IHC, IF-P, Indirect ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SERPINA5 protein
- 亚型：
IgG2a
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF-P, Indirect ELISA
- 靶点：
Protein C inhibitor
- 抗体英文名：
Protein C inhibitor Monoclonal antibody, PBS Only
- 抗体名：
Protein C inhibitor Monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
66030-1-PBS targets Protein C inhibitor in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag18094 Product name: Recombinant human SERPINA5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 106-406 aa of BC008915 Sequence: ELHRGFQQLLQELNQPRDGFQLSLGNALFTDLVVDLQDTFVSAMKTLYLADTFPTNFRDSAGAMKQINDYVAKQTKGKIVDLLKNLDSNAVVIMVNYIFFKAKWETSFNHKGTQEQDFYVTSETVVRVPMMSREDQYHYLLDRNLSCRVVGVPYQGNATALFILPSEGKMQQVENGLSEKTLRKWLKMFKKRQLELYLPKFSIEGSYQLEKVLPSLGISNVFTSHADLSGISNHSNIQVSEMVHKAVVEVDESGTRAAAATGTIFTFRSARLNSQRLVFNRPFLMFIVDNNILFLGKVNRP |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 |
| 别名 | PAI3, PCI, PLANH3, PROCI, Protein C inhibitor, SERPINA5 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC008915 |
| 基因名称 | SERPINA5 |
| Gene ID (NCBI) | 5104 |
| RRID | AB_11045661 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05154 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of ice-cold PBS containing protease inhibitor cocktail. 5. Prepare 0.2X trypsin diluted in PBS, and add 5 mL of it to the cells. Incubate in 5% CO2 for 5 min at 37°C. 6. Neutralize the trypsin with 10 mL of 10% fetal bovine
（1）体内诱生法 取Balb/c小鼠，首先腹腔注射0.5 ml液体石腊或降植烷进行预处理。1-2周后，腹腔内接种杂交瘤细胞。杂交瘤细胞在小鼠腹腔内增殖，并产生和分泌单克隆抗体。约1-2周，可见小鼠腹部膨大。用注射器抽取腹水，即可获得大量单克隆抗体。 （2）体外培养法 将杂交瘤细胞置于培养瓶中进行培养。在培养过程中，杂交瘤细胞产生并分泌单克隆抗体，收集培养上清液，离心去除细胞及其碎片，即可获得所需要的单克隆抗体。但这种方法产生的抗体量有限。近年来，各种新型培养技术和装置不断出现，大大提高了抗体的生产
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
temperature. 28. Incubate the slides overnight at 4°C in a humid chamber. 29. Rinse the slides in PBS to remove the coverslips, and place them in a rack. Wash the slides three times in blocking solution (5 min each wash
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