- ￥600 - 1200
- Proteintech
- CL750-98166
- 2025年10月09日
- FC
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
PD-L1/CD274
- 抗体英文名：
CoraLite® Plus 750 Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody
- 抗体名：
CoraLite® Plus 750 Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥600.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1200.0 |
经过测试的应用
| Positive FC detected in | anti-CD3/CD28 treated mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-98166 targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD274 antigen |
| 别名 | Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1 |
| 计算分子量 | 33 kDa |
| GenBank蛋白编号 | NM_021893.3 |
| 基因名称 | PD-L1 |
| Gene ID (NCBI) | 60533 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9EP73 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.
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文献和实验secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
x stock Tris/ Glycine SDS buffer were from National Diagnostics, (Hull, UK).eNOS (NOS 3) affinity purified rabbit polyclonal antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, Calafornia, USA).Goat Anti-Rabbit horseradish peroxidae
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
4 Antibody 22. Rabbit Anti-SeV Antibody 23. Alexa Fluor® 488 Goat Anti-Mouse IgG (H L) Antibody 24. Alexa Fluor® 594 Goat Anti-Mouse IgG (H L) Antibody 25. Alexa Fluor® 488 Goat Anti-Rabbit IgG (H L) Antibody 26. Alexa Fluor® 594
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