- ￥1500 - 3280
- Proteintech
- 84431-5-RR
- 2025年10月09日
- WB, IHC, ELISA
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
TNFSF11
- 抗体英文名：
TNFSF11 Recombinant monoclonal antibody
- 抗体名：
TNFSF11 Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | mouse spleen tissue, mouse liver tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84431-5-RR targets TNFSF11 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg1956 Product name: Recombinant Mouse TNFSF11/RANKL protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 72-316 aa of NM_011613.3 Sequence: RAQMDPNRISEDSTHCFYRILRLHENADLQDSTLESEDTLPDSCRRMKQAFQGAVQKELQHIVGPQRFSGAPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor (ligand) superfamily, member 11 |
| 别名 | 241791B7, CD254, ODF, OPGL, Osteoclast differentiation factor |
| 计算分子量 | 35KD |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | NM_011613.3 |
| 基因名称 | Tnfsf11 |
| Gene ID (NCBI) | 21943 |
| RRID | AB_3671958 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O35235 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNFSF11, known as Tumor Necrosis Factor Superfamily Member 11, also known as RANKL (Receptor Activator of Nuclear Factor-κB Ligand), TRANCE, etc., is a member of the Tumor Necrosis Factor (TNF) superfamily.RANKL is a type II homotrimeric transmembrane protein located primarily at the plasma membrane. RANKL is a type II homotrimeric transmembrane protein located mainly at the plasma membrane, and in addition to the membrane-bound form (mRNAKL), it can also be cleaved by proteolytic cleavage of the cell membrane to produce the soluble form (sRANKL). The membrane-bound form of RANKL is the main form involved in triggering the RANKL/RANK signaling pathway and mediating osteoclastogenesis. Current studies have shown that TNFSF11 acts primarily in the immune and skeletal systems and has been implicated in the pathogenesis of degenerative bone diseases, carcinogenesis, and asthma.
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文献和实验| Species | Application | Title |
|---|---|---|
Exp Cell Res Activated NETosis of bone marrow neutrophils up-regulates macrophage osteoclastogenesis via cGAS-STING/AKT2 pathway to promote osteoporosisAuthors - Yutong Guo |
ml）lml，再从鸡的翼下静脉取血0.5--1ml，取出后放入刻度离心管中，再加入2--3ml Alsver液，使总量为4--5ml，混匀并封口，置于4℃冰箱内可供一周内使用，实验时取贮备的鸡血细胞lml，加入4ml85％生理盐水，混匀后以1200rr/min离心5min，去上清液后，最后用0.6mol/L的甘露醇液(或0.6mol/L蔗糖溶液)混匀后以上述离心条件洗涤两次，最后用0.6mol/L的甘露醇液将鸡细胞稀释并调整成每毫升含2×105个。如采用传代培养的骨髓或腹水瘤细胞，可先用0.9％
是没有问题的. 2、表达重组蛋白时，细菌裂解方法都有哪些？ 在表达重组蛋白时，诱导以后跑SDS-PAGE发现表达都很好，但是在裂解细胞时遇到问题。总是不能彻底裂解细胞。 首先我采用超声裂解，可是不管我如何超声总有部分细菌没有裂解。 我的超声条件如下：宁波新芝超声细胞破碎仪，功率400W，超5秒，间隔5秒，重复99次。然后显微镜观察，不彻底时再重复99次。菌体来自200 ml培养液，用20 ml PBS重悬。 然后我又尝试加溶菌酶，首先加至终浓度100 ug/ml，4C半小时菌液不变粘
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