- ￥1350 - 3000
- Proteintech
- 32534-1-AP
- 2025年10月09日
- WB, IHC, ELISA
- Rabbit
- mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
Siglec-12
- 抗体英文名：
Siglec-12 Polyclonal antibody
- 抗体名：
Siglec-12 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32534-1-AP targets Siglec-12 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg2733 Product name: Recombinant Mouse Siglec-12 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 19-350 aa of NM_031181.2 Sequence: QNPQEVFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGDPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTNVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMM |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sialic acid binding Ig-like lectin E |
| 别名 | MIS, mSiglec-E, Myeloid inhibitory siglec, Sialic acid-binding Ig-like lectin 12, Sialic acid-binding Ig-like lectin 5 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | NM_031181.2 |
| 基因名称 | Siglec-E |
| Gene ID (NCBI) | 83382 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q91Y57 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: 22749793). Sialic acid binding Ig-like lectin 12 (Siglec-12, also known as S2V, SLG, SIGLECL1, and Siglec-XII) is a member of the SIGLEC3-like subfamily of SIGLECs, which is highly expressed in the spleen, such as CD8+ cells, macrophages, neutrophils, and dendritic cells. It mediates sialic-acid-dependent binding to cells and could be an identification of therapeutic targets and prognostic biomarkers of sarcomas (PMID: 38182638).
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